The purpose or this study was to observe the histological alteration of Langerhans cells on wound heaing process in applyoing low energy laser irradiation.
For this study, 50 Spraque-Dewly rats, weighing 150Gm or more were devided into control, experimental control group (0), 47.5Hz (1), 190Hz(3), 380Hz(5), 760Hz(7), lased group.
All the experimental animals were made excision wound on buccal mucosa, 2mm depth, and lased with stoma laser (904nm, semconductor type ASGaAl, Sedalac France) 47.5Hz, 380Hz, 960Hz, 3minutes one time respectively except experimental control
group.
After the experiment, experimental animals were sacrificed after 24hours, 48hours, 72hours on each.
Taken specimens were embedden in paraffin, sectioned 6-8u in thicness. And the langerhans cell were detected using ant S-100 protein antibcdy, and histochemically processed with Avidin Biotin complex method.
All the Langerhan cells were calculated under light microspe in 400 multiplication field and standard deviation, probability test between each group were evaluated using statistical analysis system (S. A. S) program.
@ES Following results were obtained.
@EN 1. Langerhan cells were increased in experimental control group compare to that in control group (P<0.01).
2. 24hour after experiments, Langerhans cell were decreased compare to that in control group and control experimental group 5, 1, 3.
Probability test shows significance between control experimental and 5, 1, 3 group on a=0.05 range.
3. 48hour after experiment, Langerhans cells were decreased compare to that on experimental control group, and probability test shows significance between control experimental and 3, 7, 5 group on a=0.05 range.
4. 72hour after experimentas, Langerhans cells were decreased compare to that on experimental control group and probability test on groupu comparison shows significance betwene control experimental and 1, 5 and 1 between 3, 7 between 3, and-5,
between
7, respeilively on a=0.05 range.
5. Langerhans cells number in experimental group were decreased compare to that on experimental control group in applying laser irradiation.
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